Coding

Part:BBa_K5007001:Design

Designed by: Pedro Ribeiro Faria   Group: iGEM23_USP-EEL-Brazil   (2023-09-25)


CsPT4-NoTP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 810
    Illegal SapI site found at 384


Design Notes

The main consideration for this part's design was the removal of the transport peptide, comprising of the first 77 amino acids, which should grant proper function of the synthesized enzyme in the cytosol or the plasmatic membrane. The integrity of the protein's catalytic and binding sites, and its structure was investigated through several bioinformatics analysis and tools.


Source

We got the unmodifyied sequence from iGEM22 Waterloo team, from their project Pinene Express. The original part sequence comes from Cannabis sativa's genome.

References